The stems could also have gum exuding from these areas as the bleached areas turn drier. Symptoms: Bleaching of stems and leaf death near the crown of the plant. The aim of the research was to detect a gummy stem blight resistant gene (Gsb-4) in 18 cultivar melons and 1 cultivar cucumber. Gummy stem blight (Photo courtesy of David Commens, South Pacific Seeds) Charcoal rot. It affects the leaves, stems, and fruits of all cucurbits. Therefore, understanding the resistance gene of gummy stem blight is crucial. Gummy stem blight was first reported in 1891 in France, Italy, and the United States. This study provides insight into the fine-scale genetic diversity and reproductive biology of the gummy stem blight pathogen S. Producing superior melons whose resistance of gummy stem blight (Gsb-4) is a best way to detract gummy stem blight disease. Significant population genetic structure between the two field populations demonstrated that there is regional geographic structure and limited dispersal among fields. Although dominant and widespread clones were detected, we found relatively high genotypic diversity and recombinant genotypes consistent with outcrossing. Cankers develop in the vascular tissues of the stem and a brown, gummy exudate is commonly. citrulli within and among watermelon fields, 155 isolates from two field populations in Georgia, United States were genotyped with the 18 microsatellite loci. These spots enlarge rapidly until the entire leaf is blighted. To better understand dispersal, reproduction, and fine-scale genetic diversity of S. Using the microsatellites, a rapid polymerase chain reaction-based method was developed to distinguish the three morphologically similar species causing gummy stem blight. Within this species, we found no population structure based on broad-scale geographic region or host of origin. When tested on 46 isolates from diverse cucurbit hosts and regions, the markers were robust for the dominant and widely distributed S. To improve our knowledge of gummy stem blight epidemiology, we developed 18 polymorphic microsatellite markers by combining microsatellite motif enrichment with next-generation sequencing. Sources of inoculum for epidemics, mechanisms of dispersal, and the mating system of these species are not well understood. caricae-is a devastating disease of cucurbits worldwide. Gummy stem blight and wilt are known to cause enormous losses to the global production of muskmelon (Cucumis melo). Using the microsatellites, a rapid polymerase chain reaction-based method was developed to distinguish the three morphologically similar species causing gummy stem blight. This pathogen was identified within a very short period of time by the Real-Time PCR. bryoniae was reliably identified using morphological and molecular characterization. Gummy stem blight, caused by three closely related species of Stagonosporopsis-Stagonosporopsis cucurbitacearum (syn. bryoniae as the causal agent of Gummy Stem Blight (GSB) disease of watermelon (Citrullus lanatus) in Turkey. Combining population genetics with epidemiology provides insight into the population biology of pathogens, which could lead to improved management of plant diseases.
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